Western blot. All lanes: ATP5F1D antibody at 1.94µg/ml. Lane 1: Raji whole cell lysate. Lane 2: NIH/3T3 whole cell lysate. Lane 3: A549 whole cell lysate. Lane 4: MDA-MB-231 whole cell lysate. Lane 5: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa