Western blot. All lanes: ATP5F1D antibody at 2.44µg/ml. Lane 1: Mouse heart tissue. Lane 2: Raji whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: A549 whole cell lysate. Lane 5: HepG2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 18 kDa. Observed band size: 18 kDa