Western blot. All lanes: Alpha-amylase 2B antibody at 8ug/ml. Lane 1: Mouse skeletal muscle tissue. Lane 2: HL60 whole cell lysate. Lane 3: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 58, 43 kDa. Observed band size: 58 kDa.