Western blot. All lanes: AMP deaminase 3 antibody at 2ug/ml. Lane 1: NIH/3T3 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Mouse lung tissue. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 89, 25, 77, 90, 72 kDa. Observed band size: 89 kDa.