Western blot. All lanes: Adenylosuccinate synthetase isozyme 2 antibody at 12ug/ml. Lane 1: Mouse skeletal muscle tissue. Lane 2: HepG2 whole cell lysate. Lane 3: K562 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 50 kDa. Observed band size: 50 kDa.