Western blot. All lanes: Actin-related protein 2 antibody at 3ug/ml. Lane 1: Mouse spleen tissue. Lane 2: Jurkat whole cell lysate. Lane 3: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 45, 46 kDa. Observed band size: 45 kDa.