Western blot. All lanes: ACAT1 antibody at 6µg/ml. Lane 1: Mouse skeletal musce tissue. Lane 2: Rat heart tissue. Lane 3: THP-1 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 46, 18 kDa. Observed band size: 46 kDa