Western Blot. Positive WB detected in: 10ug 293 whole cell lysate transfected with PD-1, 25ng recombinant protein from E.coli, 25ng recombinant protein from Mammalian cell . PD-1 antibody at 1:1000 . Secondary. Goat polyclonal to mouse IgG at 1/50000 dilution. Predicted band size: 72, 36 KDa. Observed band size: 72, 36 KDa. Exposure time: 5min