All lanes: Mouse anti- Human Insulin-like growth factor-binding protein 1 monoclonal antibody at 1ug/ml. Lane 1:Pyrolysis liquid human placental tissue 7.5ug/ml. Lane 2:Pyrolysis liquid human placental tissue 3.25ug/ml. Lane 3:IGFBP1 transfected SF9 cell lysate. Lane 4:human amniotic fluid. Secondary Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size : 28kd. Observed band size : 35kd.