Western Blot. Positive WB detected in: BCL2 antibody at 1:1000. Lane 1: Hela whole cell lysate. Lane 2: THP-1 whole cell lysate. Lane 3: NIH/3T3 whole cell lysate. Lane 4: MCF-7 whole cell lysate. Secondary. Goat polyclonal to Mouse IgG at 1/20000 dilution. Predicted band size: 26KDa. Observed band size: 26 KDa. Exposure time: 5min