Western Blot. Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng. All lanes: E-Tag antibody at 1:1000. Secondary. Goat polyclonal to Mouse IgG at 1/10000 dilution. Predicted band size: 36 kDa. Observed band size: 36 kDa.