MBP-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000061M0m at dilution of . Lane 1:1000 Lane 4:8000 Lane 7:64000. Lane 2:2000 Lane 5:16000 Lane 8:128000. Lane 3:4000 Lane 6:32000 Lane 9:256000. Secondary. Goat polyclonal to Mouse IgG at 1/5000 dilution. Predicted band size:50kd. Observed band size:50kd.