All lanes : Recombinant GST Protein at 30ng/ml. Line 1:Mouse Anti-GST monoclonal antibody at 1:1000. Line2:Mouse Anti-GST monoclonal antibody at 1:2000. Line3:Mouse Anti-GST monoclonal antibody at 1:5000. Line4:Mouse Anti-GST monoclonal antibody at 1:10000. Line5:Mouse Anti-GST monoclonal antibody at 1:20000. Line6:Mouse Anti-GST monoclonal antibody at 1:40000. Line7:Mouse Anti-GST monoclonal antibody at 1:80000. Predicted band size : 28kd. Observed band size : 30kd.