ZEISS Lightsheet Z.1: Sample Preparation

12 Dec 2014

This white paper describes the theoretical and practical aspects of sample preparation for Light Sheet Fluorescence Microscopy. General rules for sample handling, as well as guidelines with respect to the best preparative technique to use are presented. Step-by-step protocols, covering sample preparation ranging from micrometer-sized fluorescent beads to millimeter-sized insects, are also included.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.Sample HandlingProper and careful handling of samples is important in scientific studies to ensure the integrity of specimens. This will prevent deterioration and cross contamination, and improve the accuracy of sample tracking procedures.
ZEISS Lightsheet Z.1: Sample Preparation