Western blotting handbook

27 Jun 2021

Western blotting, also called protein blotting or immunoblotting, uses antibodies to identify specific protein targets after they have been separated by electrophoresis and transferred to a membrane. Download this handbook to find information to help you choose the right protein transfer and detection methods for your western blots.

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Mini Blot Module
Thermo Fisher Scientific
Gel blottingSpecifically designed to work with the 120 mini-vertical gel system simple and fast gel blotting with low buffer requirements and fast transfer rates. Product detail:C140 Mini Blot ModuleSpecifically designed to work with the 120 Mini-Vertical Gel System. The Mini Blot Module is a small tank blotter that incorporates the best features of a semi-dry blotter (low buffer requirements and fast transfer rates), with none of the problems associated with semi-dry blotting, such as arcing and buffer depletion. Secured by just two clamps, module assembly is simple and fast. Fast transfersFast transfers without the need for cooling when you use the Mini Blot Module. Close electrode spacing creates the high-intensity fields necessary for rapid transfers without generating excessive heat. Most blotting systems operate at 50 volts or more. This system typically operates between 15 and 25 volts to achieve transfers in as little as forty-five minutes without the need for cooling. Low Buffer VolumesEach module requires only 100 ml buffer per run compared to one liter in other commercially available mini blotters. The Mini Blot Module has a generous 8.5 x 9 cm transfer area so it accepts virtually all standard and precast mini-gels. Each module forms a self-contained chamber capable of blotting one or two gels. Two modules may be run at a time in a single outer tank, for a total blotting capacity of four gels. Mini Electroblot SystemThe Mini Electroblot comes complete with everything you need to blot two gels. It includes: one Mini Blot Module, an outer tank, the safety cover, two clamps and three Scotch-Brite™ pads. The pads are used to hold the gel and transfer membrane in place. The system may be upgraded at any time to handle four gels at once with the addition of another Mini Blot Module, pads and clamps.
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Thermo Scientific™ Pierce™ Power Blotter Welcome Pack
Thermo Fisher Scientific
The Pierce Power Blotter is designed specifically for rapid semi-dry transfer of 10-300 kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes in 5 to 10 minutes.
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Invitrogen™ XCell SureLock™ Mini-Cell and XCell II™ Blot Module
Thermo Fisher Scientific
The XCell SureLock Mini-Cell and XCell II Blot Module includes the XCell SureLock Mini-Cell for running Novex mini-gels and the XCell II Blot Module for blotting Novex mini-gels.
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Invitrogen™ iBlot™ 2 Gel Transfer Device
Thermo Fisher Scientific
The iBlot 2 Gel Transfer Device performs western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers.
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