Visualizing the Architecture of Cells and Tissues

17 Nov 2017

Laser scanning microscopes (LSMs) have proven extremely flexible in 3D imaging in a non-destructive manner. Most difficulties encountered in biological imaging are caused by intrinsic sample characteristics that compromise 3D imaging by limiting penetration depth and cause image distortions. Discriminating fluorescent labels from autofluorescence is another common challenge in plant and animal tissue samples. this application note describes how these problems can be overcome using Zeiss' LSM technology.

ZEISS Axio Imager - Modular System for Digital Fluorescence Microscopy

ZEISS Research Microscopy Solutions

Axio ImagerAn innovative modular system for digital fluorescence microscopy, featuring advanced flexibility and application versatility.  Featuring: New IC2S objectives (Infinity Contrast & Colour Corrected System) - optimise image quality and maximise contrast Special fluorescence filters - reduce exposure and image acquisition time for superior 3D imaging. 'Intelligent Stand' - automatically recognises added components.  "Contrast manager" ensures simple changes between contrasting techniques.  Integration: standard interfaces permit communication via USB and TCP/IP Vibration-free Imaging Cell - isolated within the stand for stable observation and unparalleled precision.  Apochromatic fluorescence beam path - ensures optimum colour correction Active stray light elimination - significantly higher contrast Fast, motorised reflector turrets - hold either six or ten filter modules.  HBO lamp: convenient, self-aligning homogeneous illumination LCI Plan-Neofluar objectives - live cell imaging Flexibility -  two freely configurable stand variants: Z1 (motorized) and D1(manual) or preconfigured stands M1 (motorized) and A1 (manual).  Rapid image acquisition with outstanding quality in up to 6 dimensions means more signal in less time.

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ZEISS LSM 710 for Fluorescence Imaging

ZEISS Research Microscopy Solutions

Experience freedom with your dyes and applications The new illumination and detection design of LSM 710 brings complete freedom to your fluorescence microscopy. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. LSM 710 enables confocal microscopy for a wide variety of applications. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. LSM 710 gives you flexibility, allowing you to perform confocal microscopy in exactly the way you need. Features: Oversampling with 30 percent longer sampling time per pixel for low noise Excellent contrast characteristics, even with strongly reflective samples – thanks to laser suppression that has been improved by 100-1000x A huge range of QUASAR detector configuration options – to meet your requirements The innovative grid and spectral recycling loop design ensures that not even a single valuable photon will be lost from your sample. Up to 80 percent dark noise reduction – thanks to the QUASAR array detector by Carl Zeiss 34-channel parallel imaging – across the complete wavelength range APD imaging with photon counting Low noise electronics and lasers

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Electron MicroscopyElectron microscopes (EM) are used to create high-resolution images of samples at the nanoscale by means of an accelerated beam of electrons as a source of illumination. Types of electron microscope include scanning electron microscopes (SEM), transmission electron microscopes (TEM), scanning transmission electron microscopes (STEM) and cryo-electron microscopes. Focused ion beam (FIB) microscopes are useful for modifying or milling a sample surface with nanometer precision, as well as imaging. Find the best electron microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.Digital MicroscopyDigital microscopy involves using digital cameras and sensors to capture high-resolution images of samples for analysis. It offers enhanced imaging capabilities compared to traditional optical microscopy and is widely used in biological and material science research. Explore digital microscopy systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.Software SolutionConfocal3D Imaging3D imaging technologies allow for the visualization and analysis of three-dimensional structures at high resolution. These systems are used in fields like molecular biology, material science, and medical diagnostics. 3D imaging can be applied to visualize cells, tissues, and organs, providing valuable insights into their structure and function. Browse our peer-reviewed product directory to find the best 3D imaging solutions, compare products, check reviews, and get pricing directly from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.Cell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.