ResourceLife Sciences
Visualizing Subcellular Vesicles to Quantitate Autophagy in Neuronal Cells
20 Jan 2015Autophagy can be visualized and characterized using readily available reagents. The level of staining of autophagosomes and lysosomes in live cells provides information about the mechanism of a compound’s stimulation or inhibition of the autophagy pathway. This can be accomplished using the ImageXpress® Micro XLS System, a high content screening system for widefield fluorescence or brightfield imaging of fixed or live cells, tissues, and small organisms.
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High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Neurites