ResourceLife Sciences
Visualization of Cathepsin Activity Using ProSense 680 Agent from Well to Cell to Animal
12 Dec 2012In this application note, the cathepsin-activatable ProSense 680 near-infrared (NIR) imaging agent was assessed and validated for use throughout the drug discovery process, from biochemistry to cellular assays to in vivo testing, using HT-29 colorectal cancer cells and 5-Fluorouracil (5-FU) treatment. ProSense 680 was visualized in these studies using the Operetta® High Content Imaging System and FMT® 2500 Fluorescence Tomography In Vivo Imaging System. Using the same agent in translational studies ensures that biological interactions are comparable.
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Infrared / IR SpectroscopyInfrared (IR) spectroscopy measures the interaction of infrared light with a sample, including transmission, reflectance & absorbance, facilitating the identification of analytes. Equipment used for quantitative analysis includes Fourier-transform infrared (FTIR) spectrometers, infrared cameras, FTIR gas analyzers, as well as attenuated total reflectance (ATR) accessories and pellet or film presses. Find the best IR spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Atomic Force Microscopy / Scanning Tunneling MicroscopyAtomic force microscopes (AFM) and scanning tunneling microscopes (STM) are high-resolution forms of scanning probe microscope (SPM) used to generate topological information of a sample down to the atomic scale. Instruments can generate an image of the surface topology, manipulate objects and reveal information on localized properties such as Young’s modulus, conductivity, and magnetism. High-quality STM and AFM probes optimized for your application are available, as well as other SPM-based instruments such as scanning ion conductance microscopes (SICM) & near-field scanning optical microscopes (NSOM). Find the best AFM and STM equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Drug DosageDosage is the size or frequency of the administration of a drug. Determination of the dosage of a drug is critical in pharmaceutical development to ensure the optimum balance between efficacy and toxicity.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.High Content ImagingHigh content imaging is a method combining two or more fluorescent microscopy experiments to identify substances that alter a cell’s phenotype in a desired manner. The process is adapted to multi-well plates and both the image acquisition and analysis are automated.Infrared ImagingToxicityToxicity is defined as the degree to which a substance can harm humans or animals. It is essential to study the toxicity of pharmaceutical products during the development process so that optimum dosage can be established for the administration of the drug.TumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.