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Validation of Stericup filtration systems for stem cell culture
6 Aug 2023In this application note, Merck demonstrates that microporous filtration, despite being the chosen method to prepare and minimize contamination in cell culture processes, raises apprehension about potential loss of valuable factors and addition of harmful components to growth media. This concern is particularly relevant in stem cell culture due to the cost of factors, sensitivity of cells to additives, and extended culture durations. The study reveals that using a polyethersulfone (PES) membrane-equipped Stericup® filter offers a dependable solution for worry-free expansion of embryonic stem cells, even through multiple filtration stages.
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FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UltrafiltrationEmbryonic Stem CellsStem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine.