Validation of SpectraSlide AP-1 for reliable CHO-K1 and HEK293 cell counting and viability assays

26 Nov 2025

Mammalian cell lines such as CHO-K1 and HEK293 are essential for the production of therapeutic proteins, monoclonal antibodies, and viral vectors, and are widely utilized in biopharmaceutical research and development. These cell lines are preferred for their robust protein expression, human-compatible post-translational modifications, and suitability for both transient and stable expression systems.

In this application note, Logos Biosystems evaluates the performance of the SpectraSlide® AP-1 for viability and concentration analysis of CHO-K1 and HEK293 cells using the LUNA-FX7™, and compare its results to those obtained with the PhotonSlide™.

SpectraSlide® AP-1

Logos Biosystems

The SpectraSlide® AP-1, designed for the LUNA-FX7™ automated cell counter, is an all-in-one cell counting slide that simplifies the sample prep process - saving time & money. No pipettes and no stains needed! Simply load a sample directly on the slide, insert into the LUNA-FX7™ and press COUNT.

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LUNA-FX7™ Automated Cell Counter

Logos Biosystems

The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, and precision autofocus. The LUNA-FX7™’s new features such as the Bioprocess mode, Quality Control mode, and the CountWire™ (21 CFR Part 11 compliant) software will help companies and facilities meet and surpass quality and regulatory requirements.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.
Validation of SpectraSlide AP-1 for reliable CHO-K1 and HEK293 cell counting and viability assays