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Validation And Comparison Of Single-Step Flash And Dual-Spectral Luciferase Reporter Gene Assays Using The Synergy™ Line Of Microplate Readers

Agilent Technologies
20 Oct 2013

Here we investigate the performance of a new type of dual luciferase assay using two luciferases with spectrally resolvable light emissions in either 96- or 384-well density microplates using the Thermo Scientific Gaussia-Firefly Luciferase Dual Assay or Gaussia and Renilla Luciferase Flash Assay Kits, respectively. Assay optimization was performed and assay performance was compared across several BioTek Synergy™ Multi- Mode Microplate Readers configured with injectors using lysate containing expressed luciferase enzymes.

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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.Gene Expression

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