Utilization of the Labcyte MicroClime Environmental Lid to Reduce Edge Effects in a Cell-Based Proliferation Assay
5 Nov 2018In this study, we demonstrate the benefit of utilizing the Labcyte MicroClime Environmental Lid to reduce edge effects in a functional cell-based assay by measuring the mitigation of evaporation of fluid in reaction wells. The value of using MicroClime Lids is shown for 384-well and 1536-well plates in both standard gravimetric QC assessments and functional cell based viability assays. The results demonstrate reduced evaporation, the ability to miniaturize and extend proliferation assays, while generating better growth rates, and higher quality data with reduced CV’s.
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MicroClime® Environmental Lid
The MicroClime® environmental lid incorporates a novel, fluid-absorbing matrix that minimizes edge effects and preserves the concentrations of solutions in microplate wells. The absorbed fluid creates a vapor barrier that protects the sample from the exterior environment and from evaporation, greatly reducing edge effects. The MicroClime lids also eliminate edge effects which might occur due to long-term cell incubation, such as overnight cell culture (e.g. Molecular Devices CellKey® 96 and 384 protocol). MicroClime environmental lids protect the contents of a wide variety of SBS standard microplates. Features of the MicroClime: Eliminate microplate edge effects Increase microplate efficiency by up to 20% Seamless integration Compatible with a wide range of solvents Can be filled manually or with automated bulk fillers