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Using WinNonlin with UNIFI: Enhanced Export for the Metabolite Identification Application Solution

25 Jun 2014

This application note demonstrates a simple workflow for the calculation of clearance PK values using
WinNonlin from microsomal incubations acquired and processed using UNIFI® Scientific Information System.

Screening Platform Solution with UNIFI

Waters

The only comprehensive screening solution The Waters® Screening Platform Solution with UNIFI™ is the first and only comprehensive screening solution – target, identify, quantify, and review using a streamlined analytical workflow that enables more accurate results across a wide range of applications.The platform is holistically designed, combining world-class instruments, software, Waters’ Analytical Standards and Reagents, and chemistries. All backed by Waters expert Service and Support.Based on the UNIFI Scientific Information System, the Platform Screening Solution facilitates data standardization so that information can be exchanged and transferred among departments and third-party partners. It enables all laboratory functions to work with a common backbone of analytical information including methods, processed results, and historical data and reports.Screening Platform Solution Includes: ACQUITY UPLC® I-Class System- Increased resolution and reduced peak widths result in unrivalled sensitivity.- Maximizes peak capacity to enhance MS sensitivity.- Provides the lowest carryover, complementing MS sensitivity and extending linear dynamic range.- Maximizes the benefits of sub 2-μm particle columns with the lowest dispersion. Xevo® G2-S QTof- Analyze the broadest range of compounds in the most complex samples. - StepWave™ ion optics allow for unsurpassed levels of robust sensitivity. - Delivers superior UPLC®-compatible mass resolution, matrix-tolerant dynamic range, quantitative performance, mass accuracy, and speed of analysis – simultaneously. UNIFI Scientific Information System- Consolidates state-of-the-art data acquisition, processing, and management capabilities to revolutionize screening processes. - Store, make use of, and share all data, methods, and reports. - UNIFI’s scientific library enables you to make use of your prior work, as well as the data and findings of colleagues. Analytical Standard and Reagents- Precisely formulated, ready-to-use, and will help analysts derive optimal performance from their LC and MS instruments.- Ensure quality and consistency from day-to-day, system-to-system, and lab-to-lab.

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Metabolite Identification Application Solution with UNIFI

Waters

The Waters Metabolite Identification and Application Solution with UNIFI® is built to excel in identifying and characterizing metabolites, incorporating both qualitative and quantitative tools in both drug discovery and development.The UNIFI Scientific Information System brings ease and efficiency to routine processes to enable laboratories to rapidly derive results from complex data sets. In discovery, the system helps researchers acquire answers and knowledge and more quickly so the right molecules can be selected for the pipeline, allowing laboratories to proceed or change direction with confidence. In development, it excels in unsurpassed tracking of metabolites and storage of all data surrounding the study; include pdf and Microsoft Office documents, weblinks, structures, metadata, etc., in its user-manageable scientific library.Solution consists of:ACQUITY UPLC I-Class SystemXevo G2-S QTofUNIFI Scientific Information SystemWaters Analytical Standards and Reagents

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Drug DeliveryDrug Delivery refers to dosage form, route of administration, formulations, technologies, and systems for transporting a pharmaceutical compound in the body as needed to safely achieve its desired therapeutic effect. Drug delivery is often approached via the biopharmaceutical or small molecule drug's formulation, but it may also involve medical devices or drug-device combination products. Considerations include instrumentation, software and services. MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.Pharmacokinetics
Using WinNonlin with UNIFI: Enhanced Export for the Metabolite Identification Application Solution