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Using the GX‐271 ASPEC™ for Automated Extraction of Hop Acids (Iso Alpha Acids and Organic Acids) in Beer
4 Oct 2011This application note describes the automated extraction of Hop acids by Solid Phase Extraction (SPE) for the brewing industry. Performing SPE prior to HPLC analysis of Iso‐Alpha Acids, Alpha Acids, Beta Acids, and Organic Acids is shown to be necessary to extend column life and remove particulates that could interfere with other analyses. SPE is a requirement prior to other analyses in beer such as for Free Fatty Acids, Sugars, Mycotoxins (OTA ‐ ZON), Resveratrol and Piceid, and Phytoestrogens.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BeerBeer