ResourceSpectroscopy
Using Intrinsic Tryptophan Fluorescence to Measure Heterotrimeric G-Protein Activation
21 Feb 2011This application note describes a robust automated assay system for measuring G-protein α subunit activity. This is a universal assay to measure Gαi/o activation as modulated by a variety of inputs because the assay relies on the flexible switch region SII to change conformation depending on nucleotide state. The assay is a sensitive and high-quality means to measure G-protein activation without the use of radiolabeled nucleotides.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.