ResourceLife Sciences
Using ddPCR to detect rare variant alleles in cfDNA extracted on the MagBinder Fit24 Platform
30 Jun 2025Discover how a semi-automated cfDNA extraction workflow enables highly sensitive detection of rare NRAS and EGFR mutations using ddPCR — achieving detection limits as low as 0.1% allele frequency. Extraction was achieved by employing Omega Bio-tek’s Mag-Bind® cfDNA Kit (M3298) automated on MagBinder® Fit24 Nucleic Acid Purification System. Ideal for cancer research and liquid biopsy applications.
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Mag-Bind ® cfDNA Kit
Omega Bio-TekIsolate circulating, cell-free DNA from plasma or serum using magnetic beads.
MagBinder® Fit24 Nucleic Acid Purification System
Omega Bio-TekElevate your laboratory experience with the MagBinder® Fit24 Nucleic Acid Purification System. Efficiency, affordability, precision, flexibility, and reliability, all in one powerful instrument.
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.cfDNADNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Droplet Digital PCRDroplet digital PCR (ddPCR) is a method of quantitative PCR that partitions a sample into droplets, allowing precise detection of low-abundance targets. It is commonly used in gene expression analysis and mutation detection. Explore ddPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ddPCRLiquid BiopsiesLiquid biopsies are non-invasive tests used to detect cancer and other diseases by analyzing blood or other bodily fluids. This technique is gaining popularity for early detection, monitoring treatment response, and detecting minimal residual disease. Explore liquid biopsy solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Molecular DiagnosticsMolecular diagnostics use an individual’s genetic code and gene expression to diagnose and monitor diseases. The technique is used increasingly in the field of infectious diseases and oncology, as well as areas such as coagulation, HLA typing and pharmacogenomics. Molecular diagnostics plays a pivotal role in personalized medicine.Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.