ResourceLife Sciences
Using Color Brightfield Microscopy and Image Stitching to Examine Fixed and Stained Tissues on Microscope Slides Using the Cytation™ 5 Cell Imaging Reader from BioTek®
3 Nov 2014The microscopic examination of samples and tissues is one of the most commonly used tools to investigate cellular structure of plant and animal tissues and is the hallmark of clinical diagnostic pathology. The tissues are typically stained with compounds to improve contrast resulting in colored specimens that can be optically examined. This application note describes the use of the Cytation™ 5 Cell Imaging Reader to perform color brightfield imaging on fixed and stained tissues on microscope slides.
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Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.