Use of Phase Contrast Imaging to Track Morphological Cellular Changes due to Apoptotic Activity with the Cytation™ 5 Cell Imaging Reader from BioTek

This application note demonstrates a method to incorporate automated, digital widefield phase contrast microscopy to monitor potential apoptotic effects of lead molecules. The ability to control conditions within the imaging chamber at 37 °C/5% CO₂ enabled kinetic images to be captured on an hourly basis throughout the entire incubation period. Determination of apoptotic activity was also performed with a fluorescent live cell assay. Results confirmed the validity of the image-based method to provide accurate analysis of apoptotic induction.