ResourceLife Sciences
Use of In-Fusion® Cloning for Simple and Efficient Assembly of Gene Constructs
Use of In-Fusion® Cloning for Simple and Efficient Assembly of Gene Constructs
14 Oct 2015The creation of genetic circuits and artificial biological systems typically involves the use of modular genetic components— biological parts—that can be arranged to form more complex systems. Several groups have attempted to create standardized formats to streamline the assembly of these biological parts. The BioBrick™ assembly standard was the first attempt to standardize biological parts to allow a single assembly method; it has become the basis for many similar formats. This application note demonstrates a simple and efficient method for assembling and re-engineering BioBricks without the use of restriction enzymes or ligation reactions.
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Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genetic Engineering