ResourceLab Automation
Use of Ca<sup>2+</sup> Oscillations in Primary Neuron Culture: A New HTS Model for Neuronal Diseases
15 Jan 2015Ca2+ oscillations play a physiologic role in the central nervous system and are thus involved in the study of neurological disorders. When neurons are cultured in high density in low magnesium concentration, they enter a stable firing mode mimicking epilepsy. This model, widely used in academic research, had not been characterized before in a 384 well format for MTS. This study demonstrates that AEDs are active in this assay, thus confirming its relevance in MTS. Moreover, further tests show that this model can be used to study basic anticonvulsant MOAs.
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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NeuronsNeurons are the fundamental units of the brain and nervous system, involved in transmitting information. Understanding neuron function is critical in neurobiology, neuroscience, and neurodegenerative disease research. Explore neuron research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.