Unlock miRNA Expression Profiles from FFPE Samples Using Miscript® miRNA PCR Arrays
3 Dec 2013miRNA expression profiling provides a valuable window into changes occurring in a biological system. Due to their stability and size, miRNAs can be readily extracted from FFPE samples using the miRNeasy FFPE Kit. This application note describes use of QIAGEN’s miScript PCR System to profile miRNA expression changes in total RNA samples isolated from FFPE normal and tumor lung tissues.
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miScript miRNA PCR Arrays
QIAGENThe miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96- or 384-well plates or Rotor Disc 100 arrays contain SYBR Green optimized and experimentally validated assays for either entire human, mouse, rat and dog miRNA genomes (miRNomes) or pathway-focused sets of miRNA assays. Our proprietary miRNA detection technologies enable uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. These technologies also provide the miScript miRNA PCR Array with high sensitivity, specificity, and accuracy. miScript miRNA PCR Arrays are currently available for the human, mouse, rat and dog species, and individual miRNAs can be analyzed independently with miScript Primer Assays.