ResourceLife Sciences
Ultrapure Water Free From Biologically Active Impurities Suitable for PCR
11 Jan 2011In this application note by ELGA LabWater, the requirement for ultrapure water free from contaminants that can impede DNA amplification in PCR is addressed. The need for reagents and solutions free from nucleases (DNase, RNase) which could cause breakdown of oligonucleotides is widely recognized, however, the influence of other waterborne contaminants, such as bacteria, ions and organic compounds, is rarely considered. ELGA’s PURELAB Ultra Genetic uses a multi-step purification process to produce ultrapure grade water suitable for PCR including Ultraviolet (UV) radiation and ultrafiltration.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.