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UHPLC Column and System Protection Essentials

19 Mar 2017

This application note provides information on how you can protect your UHPLC instrument and columns by investing in high-quality vials, UHPLC guards and by using filtered samples and mobile phases that will deliver optimal performance. The article provides guidance on selection of UHPLC accessories and ordering information.

SecurityGuard™ ULTRA Guard Cartridge System for UHPLC Columns

Phenomenex Inc

SecurityGuard ULTRA offers a convenient and economical way to extend core-shell, sub-2µm, and other UHPLC column lifetimes SecurityGuard™ ULTRA dramatically extends UHPLC column lifetime and is ideal for virtually any manufacturer's UHPLC, sub-2µm or core-shell columns. SecurityGuard ULTRA protects against the damaging effects of chemical contaminants and microparticulates introduced by the sample, mobile phase or system, which can negatively impact column lifetime, method sensitivity, quantitation and peak identification. By reducing the frequency of column replacements, as well as the need for troubleshooting and system down time, SecurityGuard ULTRA saves chromatographers time and money. Pressure-rated up to 20,000 psi (1,378 bar), these guard cartridges have no significant impact on chromatography, backpressure or dead volume. To watch how easy it is to install SecurityGuard ULTRA visit the product webpage. SecurityGuard ULTRA provides the following advantages: Virtually no change in chromatography Easy cartridge replacement Universal fit compatible with majority of UHPLC column endfittings Pressure rated up to 20,000 psi. Phenomenex also offers an interactive web application where users can enter their UHPLC, sub-2µm or core-shell column information to find the most appropriate column protection system. Visit the interactive selection tool at www.phenomenex.com/guardit

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.
UHPLC Column and System Protection Essentials