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Type II Pure Water Minimizes Interference in UV-VIS Spectrophotometry

ELGA LabWater
9 Oct 2011

This application note outlines the importance of the quality of the water used to prepare samples, standards and reagents for ultraviolet and visible spectrophotometric techniques. The recommendation is that pure water of at least Type II quality should be used to ensure the elimination of compounds which may interfere with the absorbance measurement of the compound or element under investigation. It was found that water used for UV-VIS analysis should have a resistivity > 1 MΩ.cm, a TOC of less than 50 ppb and have been previously microfiltered.

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ELGA LabWater

PURELAB Option systems from ELGA are the very best solution for producing pure water (type II) They offer a wide range of flow-rates to suit your needs, advanced purification technologies to provide pure water on demand, plus microprocessor control for consistent quality. Different laboratory applications demand different water purity levels. ELGA offers a choice of general laboratory grade water systems in the PURELAB Option range to cover applications ranging from glassware washing and the feeding of ultra-pure water systems to cell culture and media preparation. The PURELAB Option-S series delivers better than single-distilled quality, whilst the PURELAB Option-R and E series produce better than double-distilled quality.

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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.

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Type II Pure Water Minimizes Interference in UV-VIS Spectrophotometry