Tumor Histoids: Scalable Production of Living Human Mini‐Tumors for High Throughput Drug Screening
10 Sept 2013Tumor Histoids (TH) are living three‐dimensional human mini‐tumors consisting of stroma invaded by tumor cells. They can be produced in quantity and format suitable for high throughput screening. Stromal‐epithelial interactions occur spontaneously during TH culture and model those that activate pathways controlling tumor growth, malignant progression and response to therapies in vivo. This report describes the adaptation of the original TH production method to production in Perfecta™ hanging drop plates, as well as an experiment comparing cisplatin effect on TH, tumor cell spheroids and monolayers.
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Perfecta3D Hanging Drop Plates - 384-Well
3D BiomatrixThe patent-pending Perfecta3D® Hanging Drop Plates are 96- and 384-well plates that facilitate the formation, culture, and testing of three-dimensional (3D) spheroids and stem cell aggregates without the aid of coatings or matrices. With only one spheroid forming in each well, 3D spheroids formed in Perfecta3D Hanging Drop Plates are consistent and diameter can be controlled by the number of cells seeded in each well. Media and compounds can be added or removed from the top of the plate, and cells can be added for patterned co-cultures. The well plate format means that the plates are compatible with manual or automated liquid handling equipment. Product and ordering information can be found at www.3DBiomatrix.com/products.
Perfecta3D Hanging Drop Plates - 96-Well
3D BiomatrixThe patent-pending Perfecta3D® Hanging Drop Plates are 96- and 384-well plates that facilitate the formation, culture, and testing of three-dimensional (3D) spheroids and stem cell aggregates without the aid of coatings or matrices. With only one spheroid forming in each well, 3D spheroids formed in Perfecta3D Hanging Drop Plates are consistent and diameter can be controlled by the number of cells seeded in each well. Media and compounds can be added or removed from the top of the plate, and cells can be added for patterned co-cultures. The well plate format means that the plates are compatible with manual or automated liquid handling equipment. Product and ordering information can be found at www.3DBiomatrix.com/products.