ResourceLife Sciences
TRP’ing on QPatch in Mulitmode Mode
30 Mar 2011In this application note three different transient receptor potential ion channels (TRPA1, TRPV1 & TRPM8) are tested in both single-hole mode and in multihole mode patch clamping with different agonists and antagonists. The advantages of testing compounds in multihole mode will be a minimization of biological variance and an increase in current amplitude, since the current response from several cells are summarized. The disadvantage is lack of serial resistance compensation and in some cases large leak currents.
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QPatch HT and QPatch HTX
Sophion BioscienceThe QPatch HT and QPatch HTX enable high-throughput patch clamping with 48 parallel recording sites. Automated patch clamping with the same high quality afforded by conventional patch clamping can now be applied in all phases of ion channel drug discovery, including primary screening of focused compound libraries. • Up to 7000 data points per day • Screening and compound profiling • Multi-hole and single-hole mode (QPatch HTX) • Ligand- and voltage-gated recordings • Eight pipette liquid handling with intelligent scheduler • True gigaseals • Flow channels for solution exchange • Up to 50 liquid additions • User-friendly experiment execution • Unattended operation for several hours • On-board cell preparation • Elevator units for QPlate stacking • Elevator units for compound plate stacking • Cell clone screening feature • Multiple intracellular solutions testing feature • User friendly assay setup and quick data analysis
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