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Troubleshooting LC-MS/MS Peak Shape and Recovery Problems for Polar Opiates
11 Dec 2017While supporting multiple research laboratories developing reversed-phase LC-MS/MS methods to analyze diluted urine samples for polar opiates such as morphine, normorphine, hydromorphone and morphine glucuronides, several instances of peak fronting were encountered, peak splitting as well as poor recoveries as some of the analyte molecules eluted as “breakthrough” peaks far ahead of their expected retention times.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OpiatesMorphine