ResourceLife Sciences
Transmembrane Potential Measurements in Cardiac Micro Tissues Derived from Human Stem Cells
24 Sept 2015The electrical characteristics of mammalian adult myocardium depend critically on the geometry of the tissue. Production of large quantities of human cardiac myocytes differentiated from induced pluripotent stem cells (hiPSC-CMs) is now available commercially. These cells, normally cultured on 2D surfaces, are increasingly used to assess the electrophysiological effects of drugs. This study examined the electrical activity of a commercially available hiPSC-CM cell line in both 2D culture and in 3D culture of micro-tissues.
Links
Tags
Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Microplate StackerCell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.Microtissue