Transcreener® ADP2 Assay: Simplifying and Streamlining Kinase Profiling from Experimental Design through Data Analysis

ADP detection is an attractive approach for screening kinases and other ATP-utilizing enzymes because it provides a universal platform that can be used for any member of the kinase superfamily as well as many other ATP-dependent enzymes, regardless of the acceptor substrate. The Transcreener ADP2 Assays use direct immunodetection of ADP with three different fluorescent detection modes (FP, TR-FRET and FI) to provide a highly sensitive and versatile HTS method for kinases and other ATP-utilizing enzymes. It is the only ADP detection method that does not rely on the use of coupling enzymes to convert ADP to a secondary analyte, which makes it less prone to interference from screening compounds. In this poster, the available ADP detection methods with respect to assay principle, protocol, and performance are compared. The poster also highlights the approach that has been developed at BellBrook Labs to streamline kinase assay development with the Transcreener ADP Assay, with an emphasis on minimizing experimental time and reagent consumption and simplifying data analysis.