ResourceLife Sciences
Total RNA Extraction from Mouse Kidney Before RNA Sequencing
RNA Extraction from Mouse Kidney for RNA Sequencing
21 Oct 2018Gene expression levels in an organ are only reliably estimated if extraction is performed from the whole organ (as smaller parts may not be representative of the whole organ). This application note demonstrates the best protocol for RNA extraction using the Precellys® 24, which provides a very efficient extraction from either a half or a whole mouse kidney. The researchers also compared the RNA quality and integrity after homogenization.
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA-SeqRNA sequencing (RNA-seq) is a next-generation sequencing technique used to analyze the transcriptome, providing insights into gene expression and regulation. This method is essential for genomics, disease research, and personalized medicine. Explore RNA-seq tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.