ResourceLife Sciences
Time Savings: Image Acquisition and Preparation for Publication
6 May 2015In this case study, Dr Dawn Dawson, Assistant Professor at Case Western Reserve University's Institute of Pathology, evaluates the use of ChromaCal in her research. She conducted a study to evaluate four antibodies for immunohistochemical staining. Images for each antibody were taken and an assessment of the steps taken and time required to bring consistency to the images is given. This was then followed by a comparison of the process and time needed to achieve the same level of consistency using ChromaCal.
Links
Tags
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bright Field Microscopy