Thermo Scientific Solaris RNA Spike Control Kit: Identifying Reaction Inhibition in the RT-qPCR Workflow

20 Dec 2013

This application note describes the utility of the Solaris RNA Spike Control Kit to identify reaction inhibition and provide a complementary technology to better understand and interpret RNA quality. Experimental results demonstrate the Spike Control Kit accurately identifies RT-qPCR inhibition in the presence of heparin, lysis buffer and phenol.

Solaris qPCR Expression Assays

Thermo Fisher Scientific

Pre-designed, gene-specific probe and primer pairs for repeatable, sensitive and gene specific RT-qPCR quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs). Thermo Scientific Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB) and Superbase technologies to deliver repeatable, sensitive and gene specific RT-qPCR (real-time) quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs).Solaris Assays are pre-designed to specifically detect all known variants of a gene with a single assay for high-confidence results. Solaris qPCR Primer and Probe Sets should be used in conjunction with the complementary instrument-specific Solaris qPCR Gene Expression Master Mix for optimal performance.Solaris qPCR Assays have also been pre-designed to the most commonly used reference genes to help you normalize expression levels of your experimental samples.Solaris qPCR Expression Assays Features & Benefits: High performance assay – Expect excellent results in terms of repeatability, sensitivity, dynamic range, efficiency and specificity. Sequence information included – Probe and primer sequence information is provided with every assay so you can publish with confidence. The master mix is blue – Solaris assays have been developed with our optimized, blue qPCR master mix for ultimate assay performance and ease-of-use. See your reagents as you pipette, track your progress across multiple wells, and visually assess your set-up. Familiar protocol – If you currently use qPCR probe assays, Solaris Assays will not require major changes to your protocol or routine.

(0)

Links

Tags

PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Thermo Scientific Solaris RNA Spike Control Kit: Identifying Reaction Inhibition in the RT-qPCR Workflow