ResourceLife Sciences
Therapeutic nucleic acid application notebook
10 Sept 2023Oligonucleotide synthesis includes the repeated use of protection groups that need to be removed after the last cycle. Impurities emerge through the faulty addition of two similar nucleotides in one cycle or a failed coupling of the last nucleotide. Chromatography can also separate oligonucleotides carrying protection groups from deprotected oligonucleotides and it can even facilitate the cleavage and removal of protection groups. In this notebook from Tosoh Bioscience, explore several applications for the purification of synthetic oligonucleotides.
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TSKgel® DNA-NPR weak anion exchange column
Tosoh Bioscience - SeparationsThe TSKgel DNA-NPR weak anion exchange column is designed to analyze nucleic acids. It routinely separates plasmid isoforms, PCR products and oligonucleotides.
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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.