The Tip of the Iceberg: How Pipette Tips Influence Results

3 Mar 2016

This application note demonstrates the influence of pipette tip quality in the production of accurate pipetting results. The standard ISO 8655:2002 recommends using pipettes and tips from the same supplier, but as pipette tips are only consumables, they are not usually selected with quality in mind. As a result, consumables can have a direct impact on the quality of results. Problems with analysis results are usually linked to reagents, method and pipette but not to the pipette's consumable for example. Eppendorf standard tips have been shown to perform within permissible error tolerances regardless of the calibration method or autoclaving. Here, the user is free to choose the method most suitable for his application.

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There are certain situations in the lab in which suitable safety precautions must be taken in counteract any potential risks and to prevent any unpleasant surprises. It must be possible to anticipate in advance the dangers presented by carryover effects from radioactive, highly toxic or infectious sample material as well as the contamination risk caused by foreign DNA (e.g. as a result of aerosol formation).Our ep Dualfilter T.I.P.S. have an integrated dual filter made of pure hydrophobic polyethylene without "self-sealing" additives. This filter provides protection against carryover and contamination caused by aerosols and does not swell up as a result of incorrect pipetting. Samples can then be easily recovered. In line with the special biological purity requirements of a PCR application, ep Dualfilter T.I.P.S. are "PCR clean", sterile (in accordance with USP, DAB and Ph.Eur.) and thus free of human DNA, DNase, RNase and PCR inhibitors. The new Eppendorf ep Dualfilter T.I.P.S. are the first filtertips with a two phase filter for contamination protection. The tips are manufactured to the highest possible quality from pure, non recycled materials under cleanroom conditions. The are characterized by defined flow dynamics, low wettability and high thermal stability. They are certified pyrogen free and PCR clean (free from human DNA, DNase, RNase and PCR-inhibiting substances). The two-phases effect! Two filter layers provide dual protection for pipette and sample Two filter layers provide dual protection from aerosols and biomolocules Free from PCR inhibitor additives Patent pending technology All tips are provided sterile, Eppendorf PCR clean and pyrogen-free Batch certified ep Dualfilter T.I.P.S. contains well-defined different pore sizes within the two filter layers. The white layer which faces the sample retains drops, splashes and aerosols. the blue layer above it forms a highly-effective double barrier, that reliably binds even biomolecules. Even more, ep Dualfilter T.I.P.S. are characterized by: Defined flow dynamics Low wettability High thermal stability Leak proof seal* Minimum attachment and ejection forces* *when used in combination with Eppendorf pipettes
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Labware and Lab SuppliesAutomation Supplies / AccessoriesAutomated Liquid HandlingPipette CalibrationQuality ControlQuality TestingElectronic PipetteLab EssentialsProcess OptimizationPipette Tips

Pipette tips - ep Dualfilter T.I.P.S.

Eppendorf

There are certain situations in the lab in which suitable safety precautions must be taken in counteract any potential risks and to prevent any unpleasant surprises. It must be possible to anticipate in advance the dangers presented by carryover effects from radioactive, highly toxic or infectious sample material as well as the contamination risk caused by foreign DNA (e.g. as a result of aerosol formation).Our ep Dualfilter T.I.P.S. have an integrated dual filter made of pure hydrophobic polyethylene without "self-sealing" additives. This filter provides protection against carryover and contamination caused by aerosols and does not swell up as a result of incorrect pipetting. Samples can then be easily recovered. In line with the special biological purity requirements of a PCR application, ep Dualfilter T.I.P.S. are "PCR clean", sterile (in accordance with USP, DAB and Ph.Eur.) and thus free of human DNA, DNase, RNase and PCR inhibitors. The new Eppendorf ep Dualfilter T.I.P.S. are the first filtertips with a two phase filter for contamination protection. The tips are manufactured to the highest possible quality from pure, non recycled materials under cleanroom conditions. The are characterized by defined flow dynamics, low wettability and high thermal stability. They are certified pyrogen free and PCR clean (free from human DNA, DNase, RNase and PCR-inhibiting substances). The two-phases effect! Two filter layers provide dual protection for pipette and sample Two filter layers provide dual protection from aerosols and biomolocules Free from PCR inhibitor additives Patent pending technology All tips are provided sterile, Eppendorf PCR clean and pyrogen-free Batch certified ep Dualfilter T.I.P.S. contains well-defined different pore sizes within the two filter layers. The white layer which faces the sample retains drops, splashes and aerosols. the blue layer above it forms a highly-effective double barrier, that reliably binds even biomolecules. Even more, ep Dualfilter T.I.P.S. are characterized by: Defined flow dynamics Low wettability High thermal stability Leak proof seal* Minimum attachment and ejection forces* *when used in combination with Eppendorf pipettes

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The Tip of the Iceberg: How Pipette Tips Influence Results

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