SelectScience
Join Free
Accelerating Science
ResourceSeparations

The Samplicity® Filtration System Streamlines Chromatography Sample Preparation

Merck
20 Jun 2011

In this application note EMD Millipore compare sample filtration, prior to chromatographic separation, through hydrophilic polytetrafluoroethylene (PTFE) MillexR syringe filters with a 10 mL syringe to 0.45 µm hydrophilic PTFE Millex Samplicity® filters using the Samplicity filtration system. EMD Millipore’s Samplicity sample preparation system enables filtration of up to 8 samples directly into standard HPLC vials using vacuum driven filtration. The filtration speed for three different samples with varying composition and viscosity and cross-contamination of filtrates when processing multiple samples at one time are evaluated.

Related products

Request Quote for All Products

Samplicity® filtration system

Merck

Introducing a vacuum filtration system that makes sample preparation easy: the EMD Millipore Samplicity filtration system with Millex Samplicity membrane filters. With the Samplicity® filtration system and Millex Samplicity® membrane filters, EMD Millipore has transformed sample preparation forever. Advantages of the EMD Millipore Samplicity filtration system include:   •  Higher throughput with simultaneous vacuum filtration of up to eight samples   •  Ergonomic alternative to syringe filters   •  Easy sample preparation of even highly viscous or particulate-laden mixtures   •  Millex Samplicity membrane filters provide long-trusted quality with low extractables and low analyte binding   •  Choose the unit color to fit your lab—bold blue or glossy green Applications of the EMD Millipore Samplicity filtration system include sample preparation for:   •  Dissolution testing   •  High-pressure liquid chromatography (HPLC)   •  Ultra-high performance liquid chromatography (UHPLC or UPLC®)  •  Liquid chromatography – mass spectrometry (LCMS)

(1)

Links

MerckCompany website

Tags

UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.

Related resources

The Samplicity® Filtration System Streamlines Chromatography Sample Preparation