ResourceLife Sciences
The reconstruction and immunocytochemical characterization of polarised epithelia in ThinCert cell culture inserts
19 Feb 2024In this application note, Greiner Bio-One GmbH presents ThinCert® inserts for biologically relevant in vitro approaches as alternatives to animal experimentation. The note highlights specific protein localization to demonstrate epithelial polarization, showcasing the effectiveness of ThinCert® inserts in reconstructing epithelia and restoring biological functions in vitro.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. 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