The MED64 Multi‐Electrode Array System

19 Nov 2014

The MED64 has become a popular platform for modeling human hereditary cardiomyopathies and drug screening, since hSC-CMs possess the same genetic background as primary human CMs and their pharmacology is presumably very similar. The goal of this application note is to describe how to set up experiments with human stem cell-derived cardiomyocytes (hSC-CMs), acquire relevant data, and extract the data for presentation or publication.

MED64 Multi-electrode Array System

AutoMate Scientific Inc.

Bandwidth of 0.1Hz -10 kHz allows users to record several types of extracellular potentials. • Low-cut filter (High pass filter) with 0.1, 1, 10, and 100Hz cutoff. • High cut filter (Low pass filter) with 1, 2.5, 5, 7.5, and 10kHz cutoff. • Built-in 2-channel stimulator allows users to program and deliver complex stimulation sequences independently through any 2 electrodes. • USB connection to computer.SpecificationsHead Amplifier Number of channels 64Gain x 10Bandwidth 0.1Hz-100 kHz (+0dB to -3dB)Input impedance 100MΩOutput impedance 10kΩ Stimulator Number of channels 2Output format: Constant currentMaximum input voltage +/-4VMaximum output current +/-200µA ---Main Amplifier Number of channels 64Gain 1-217Bandwidth 0.1Hz-10 kHz (+0dB to -3dB)Analog high cut filter 1, 2.5, 5, 7.5, 10kHz (-6dB/oct)Analog low cut filter 0.1, 1, 10, 100Hz (-12dB/oct)Input impedance 100 MΩ Digitizer Resolution 16 bitSampling rate 20kHzOutput: USB

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MED64 "Mobius" MEA software - Pro, complete package

AutoMate Scientific Inc.

MED64 Mobius software is a data acquisition and analysis package for the MED64 multi-electrode array system with an abundant set of analysis functions available both online and offline. It is designed to be easy for beginners and powerful for advanced users. Mobius comes in various packages including “Evoked Potential Measurements” and “Spike Sorter” as well as a combined package.Mobius is flexible software consisting of various task-specific control panels which users can quickly combine using a simple workflow editor to create their own custom experimental protocols and interfaces. However, users who want to run standard types of experiments can do so immediately by using one of the many pre-defined “workflow templates” included with Mobius.EP (Evoked Potential) Measurement PackageSpike Sorter PackageFiltering Module

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ElectrophysiologyElectrophysiology is the study of biological voltage changes, from a single ion channel through to a whole organ scale. Voltages can be measured using patch clamping, automated workstations, electrophysiology rigs or cell based assays. Specific cell lines, manipulators and microinjectors are also available for electrophysiology research.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Human Cardiomyocytes
The MED64 Multi‐Electrode Array System