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The Importance of Reliable and Efficient Sample Preparation in the Development of a Novel Forensic Method to Detect Diclofenac Residuesin Vultures and Livestock Animals

10 Sept 2015

A technique was developed to identify diclofenac residues in vultures and livestock animals by GC-MS. This paper reviews the development of this methodology, the technical challenges faced, with emphasis on sample preparation, and the role of the miVac sample condenser in increasing the overall efficiency and reliability of the method and its validation.

miVac Concentrators

Genevac

miVac is a range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. miVac concentrators come in 2 sizes, the smaller Duo and a larger Quattro. There is a choice of vacuum pump and rotor according to application and sample format.  To speed concentration built-in special runs for working with water and water mixtures improve performance, helping optimise concentration time.  The addition of the revolutionary SpeedTrap condenser to the system will greatly enhance concentration performance.  miVac systems feature unique JetRotors - solid aluminium rotors which speed concentration manifold. An optional pressure control unit provides display of chamber pressure and manual or automatic control of the vacuum level. The miVac SpeedTrap can also be used for freeze drying when configured with a miVac Super vacuum pump, as a combined concentration / freeze drying workstation with a miVac vacuum concentrator or as a stand-alone freeze drier. Applications: Suitable for use with Oligo-synthesis, RNA/DNA prep., peptide prep., sequencing, molecular biology, ADME / toxicology labs, process development, residue analysis, environmental analysis and general academic/life science research.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.GC-MS GC-MS (gas chromatography-mass spectrometry) instruments and equipment are used to separate, quantify and identify mixtures of small and volatile compounds, such as polycyclic aromatics, fatty acids and alcohols. Often used in drug detection, forensic investigation and environmental analysis for pesticides and contaminants, GC-MS is a powerful addition to your lab’s analytical capabilities. GC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzers can give greater sensitivity and resolution to your analysis. Find the best GC-MS instruments and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ConcentrationSample concentration involves the removal of solvents from samples using technologies such as centrifugal evaporation, lyophilization and rotary evaporation.