SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

The Importance of Coverage Uniformity Over On-Target Rate for Efficient Targeted NGS

Twist Bioscience
23 Jun 2019

In this paper, Twist Bioscience use ranges of on-target rate and uniformity typical of commercial exome kits to mathematically model the relative impacts of both metrics on overall efficiency. It's demonstratd that, though most commercial probe panels cite only on-target rate in their specifications, uniformity has a more significant contribution to the efficiency of targeted sequencing.

Related products

Request Quote for All Products

Twist Human Core Exome Kit

Twist Bioscience

Twist Bioscience, the leader in synthetic DNA with unparalleled precision at scale, is redefining targeted sequencing performance with the Twist Human Core Exome Kit. This kit includes library preparation and enrichment components, allowing the flexibility to lower sequencing costs, increase sample throughput, and achieve a higher depth of coverage across target regions with uncompromising quality.

(3)

Links

Twist BioscienceCompany website

Tags

Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.

Related resources

The Importance of Coverage Uniformity Over On-Target Rate for Efficient Targeted NGS