The Importance of a Stable Fluorescence Light Source in FRET Measurements
26 Apr 2011This application note reviews a few of the most relevant sources of error in FRET-based measurements and point out how having a stable and precisely controllable fluorescent light source, like that offered by the X-Cite® exacte, can help to reduce these complications by providing stable fluorescence illumination. The FRET ratios in HEK cells using two light sources with differing output stability are compared.
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X-Cite Exacte
Lumen DynamicsThe X-Cite® exacte employs a DC-powered mercury lamp coupled with patented Closed-Loop Feedback technology to provide ultra-stable illumination, for imaging protocols lasting from milliseconds to days. With the X-Cite® Optical Power Measurement System, light output can also be calibrated in absolute power units (watts), ensuring truly repeatable experiments. Features of the X-Cite® exacte: Lamp: 200W, Pre-aligned, Intelli-Lamp® technology, 2000 hour guaranteed life. Includes: Closed-Loop Feedback technology, Adjustable iris in 1% increments, Computer Control & GUI, Internal high speed shutter. Compatible with Carl Zeiss, Leica, Nikon, Olympus and Motic microscopes. Additional Specifications of the X-Cite® exacte: Certifications: CE Marked, Certified to IEC, Canadian and US Standards